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Vol. 15 No. 1 (2024)
DOI: 10.59987/ads/2024.1.9-16
Articles
2024-03-28

In Vivo Biocompatibility and Biodegradation Test of Two Barrier Membranes for Guided Tissue Regeneration

Authors

Machiavelli Clavesia, Stephani Dwiyanti, Veronika Maria Sidharta, Dyonesia Ary Harjanti, Eko Adi Prasetyanto, Tena Djuartina

Keywords: Biocompatibility, Biodegradation, In Vivo, Polycaprolactone, Guided Tissue Regeneration

Abstract

This study evaluates biocompatibility and biodegradation properties of PCL/Chitosan/BG-NP/Tetracycline and compares them with commercially available SureDerm membrane in Sprague Dawley Rats.Two different membranes (PCL/Chitosan/BG-NP/Tetracycline membrane and SureDerm membrane) were randomly inserted into subcutaneous pouches in the backs of 54 Sprague Dawley rats. The animals were sacrificed at day 7, 21, and 63. Biocompatibility properties (number and distribution of inflammatory cells, necrosis, neovascularisation, fibrosis, fatty infiltrate, tissue integration, tissue ingrowth) was assessed according to DIN EN ISO 10993-6. Biodegradation property was assessed macroscopically to check for membrane degradation rate. In terms of biocompatibility properties, the test membrane had overall irritancy score of 0 at day 7 and 21, and 0.66 at day 63, and it is considered non-irritant. Biodegradation of test membrane is faster compared to SureDerm at day 7 and 21. However, there is no significant difference between the two membranes at day 63. The GTR membrane PCL/Chitosan/BG-NP/Tetracycline is a good membrane with comparable biocompatibility and biodegradation properties to the SureDerm membrane.

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Authors

Machiavelli Clavesia - School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Pluit Raya No. 2, North Jakarta, 14440

Stephani Dwiyanti - Department of Dental Medicine, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Pluit Raya No. 2, North Jakarta, 14440

Veronika Maria Sidharta - Department of Histology, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Pluit Raya No. 2, North Jakarta, 14440

Dyonesia Ary Harjanti - Department of Anatomical Pathology, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Pluit Raya No. 2, North Jakarta, 14440

Eko Adi Prasetyanto - Department of Pharmacy, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Pluit Raya No. 2, North Jakarta, 14440

Tena Djuartina - Department of Biomedical and Anatomy, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Pluit Raya No. 2, North Jakarta, 14440

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors contributing to Annali di Stomatologia agree to publish their articles under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which allows third parties to copy and redistribute the material providing appropriate credit and a link to the license but does not allow to use the material for commercial purposes and to use the material if it has been remixed, transformed or built upon.

How to Cite
Clavesia, M. ., Dwiyanti, S. ., Sidharta, V. M. ., Harjanti, D. A. ., Prasetyanto, E. A. ., & Djuartina, T. . (2024). In Vivo Biocompatibility and Biodegradation Test of Two Barrier Membranes for Guided Tissue Regeneration. Annali Di Stomatologia, 15(1), 9–16. https://doi.org/10.59987/ads/2024.1.9-16